What is the functional group used in DEAE resin?
diethylaminoethyl group
Price Inquiry
| Cat.no : | diethylaminoethyl-001D |
|---|---|
| Ion exchange capacity : | 3–4 mmol/g dry powder |
| Functional group : | diethylaminoethyl group:–O–CH2CH2–N+(C2H5)2H |
| Average particle size : | 40-120 μm |
| Ligand : | Diethylaminoethyl |
What is DEAE column?
SMT DEAE [Di-Ethyl-Amino-Ethyl] column provides a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirable for the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’s and plasmid DNA’s.
What is DEAE Sepharose chromatography?
Overview. DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification. Well-proven weak anion exchanger developed for industrial downstream processes. Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
Is DEAE cellulose anion or cation exchanger?
At neutral pH the carboxymethyl group is ionized as -CH2OCH2COO¯ so that CM- cellulose is negatively charged, so it is a weak cation exchanger. DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger.
What does DEAE stand for?
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
How does DEAE chromatography work?
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations).
What is DEAE cellulose resin?
DEAE cellulose or DE 52 is a preswollen weak anion exchange resin (Diethylaminoethyl cellulose) that provides high binding capacity, clean resolution and ease of use over dry forms of DEAE cellulose. This DEAE 52 cellulose is typically used for ion-exchange chromatography of proteins and nucleic acids.
How does DEAE cellulose work?
DEAE cellulose is an anionic resin which can be used to bind -vely charged proteins. Proteins can be eluted by altering either by increasing the salt concentration or by altering the pH. DEAE is a weak ion exchange resin, which means the pH range at which it can be used is limited, charge density will vary.
What is the meaning of DEAE?
Noun. 1. DEAE cellulose – used for chromatography. diethylaminoethyl cellulose. cellulose – a polysaccharide that is the chief constituent of all plant tissues and fibers.
Which resin is used in ion exchange chromatography?
Anion resins and cation resins are the two most common resins used in the ion-exchange process. While anion resins attract negatively charged ions, cation resins attract positively charged ions.
Why resin is used in ion exchange chromatography?
Ion exchange resins are used to soften water by replacing the cations with sodium ions (and possibly the anions with chloride ions) of sodium chloride. They may also be used to demineralize water where the cations are replaced by H+ ions and the anions are replaced by OH− ions.
What are the different types of ion-exchange resins?
Ion exchange resins consists of two main types, i.e., cation exchange resins, that exchange positively charged ions, such as sodium, for calcium, and anion exchange resins, that exchange negatively charged ions, such as chloride, for arsenic.
How do you choose ion exchange resin?
Selecting Ion-Exchange Resins The internal structure of the resin beads, i.e., whether microporous (gel-type) or macroporous, is important in the selection of an ion exchanger. Macroporous resins, with their high effective surface area, facilitate the ion exchange process.
What are the different grades of resin?
Resins are categorized by family and type that fall into three different grades; commodity, engineering, and specialty/high-performance. When selecting a plastic to use, you should begin by choosing the appropriate grade of material that has the performance requirements your finished product must meet.