Is vimentin a tumor marker?
Vimentin also has an important clinical significance as a tumor marker. All intermediate filament proteins are expressed in a highly developmentally-regulated fashion; Vimentin is the major cytoskeletal component of mesenchymal cells.
What is vimentin in cancer?
Vimentin (VIM) is a type III intermediate filament that maintains cell integrity, and is involved in cell migration, motility and adhesion. When overexpressed in solid cancers, vimentin drives epithelial to mesenchymal transition (EMT) and ultimately, metastasis.
Are MDA MB 435 cells HER2 positive?
Interestingly, the two cell lines used in the initial cell-line studies of BRMS1 were both ER, PR negative; and MDA-MB-435 was HER2 positive in the current study.
What does vimentin positive mean?
Mesenchymal and endothelial cells usually stain vimentin positive and thus act as a measure of internal quality control in immunoreactivity. Absence of immunostaining in these areas often indicates that there is significant damage to tissue antigens and loss of structural architecture.
What tumors are vimentin positive?
Vimentin is overexpressed in various epithelial cancers, including prostate cancer, gastrointestinal tumors, tumors of the central nervous system, breast cancer, malignant melanoma, and lung cancer.
Do MDA-MB-231 cells express HER2?
MDA-MB-231 is a highly aggressive, invasive and poorly differentiated triple-negative breast cancer (TNBC) cell line as it lacks oestrogen receptor (ER) and progesterone receptor (PR) expression, as well as HER2 (human epidermal growth factor receptor 2) amplification2,3.
What is the difference between MDA-MB-231 and MDA MB 468?
MDA-MB-468 is a basal BC cell line while MDA-MB-231 is a claudin-low cell line – a point Hamed Helal made above, to his credit – with claudin-3, claudinin-4 and claudinin-7 low, and typically with low Ki67 and E-cadherin, in contrast to MDA-MB-468 absent these and itself with high Ki-67 and as I noted, MDA-MB-231 shows …
What is the doubling time for MDA-MB-231?
The doubling times for exponentially growing MDA-MB-231 cells were 28.9 h, whereas the doubling times of the eight microcell hybrids varied between 33.4 h (CT60/4) and 44.6 h (CT60/8) (medium 37.7 h) (Figure 2a).
Is vimentin positive in melanoma?
The data showed that high expression of vimentin was significantly associated with melanoma hematogenous metastasis.
What does a positive vimentin mean?
What is the difference between MCF7 and MDA-MB-231?
The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.
Does vimentin regulate cytoskeleton architecture regulation in low malignant breast cancer cells?
To identify the role of vimentin in cytoskeleton architecture regulation, the low malignant breast cancer cell MCF7 was transfected with a PSmOrange vector that was carrying vimentin. Figure Figure6A6Ashows the protein levels of vimentin overexpressed in MCF7 cells.
What is the MDA-MB-231 cell line?
This cell line is ER, PR, and E-cadherin negative and expresses mutated p53. In microarray profiling, the MDA-MB-231 cell genome clusters with the basal subtype of breast cancer.
What is the role of vimentin in epithelial cell adhesion and motility?
Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J. 2010;24:1838–1851.
Does vimentin regulate intracellular mechanical homeostasis in EMT cancer cells?
The EMT-related transcription factor slug was also mediated by vimentin. The current study demonstrated that vimentin serves as a regulator to maintain intracellular mechanical homeostasis by mediating cytoskeleton architecture and the balance of cell force generation in EMT cancer cells.