What is PIN1 protein?
PIN1 is a member of a family of peptidylprolyl isomerases that bind phosphoproteins and catalyze the rapid cis–trans isomerization of proline peptidyl bonds, resulting in an alteration of protein structure, function, and stability.
What is an example of a recombinant protein used therapeutically?
The first recombinant protein used in treatment was recombinant human insulin in 1982. The recombinant protein industry has rapidly grown. To date, more than 130 recombinant proteins are approved by the US FDA for clinical use.
What proteins are produced in E coli?
Introduction. The bacterium Escherichia coli is one of the most widely used hosts to produce recombinant proteins (Rosano and Ceccarelli, 2014). While the majority of recombinant proteins are produced in the cytoplasm of E. coli, it is common to produce recombinant proteins that contain disulfide bonds in the periplasm …
Why is E coli used for protein production?
coli strain (K strain), E. coli BL21 (B strain) is the most used for recombinant protein production because B strains lack some proteases, achieve higher biomass yields and produces much less acetate than E.
What is the function of Pin1?
Peptidyl-prolyl cis-trans isomerase Pin1, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Rotamase Pin1 and PIN1, peptidyl-prolyl cis/trans isomerase (PPIase), is a nucleus protein. PIN1 is a peptidyl-prolyl isomerase that can alter the conformation of phosphoproteins and so affect protein function and/or stability.
What is the molecular mass of Pin1?
The recombinant human PIN1 consisting of 179 amino acids and has a calculated molecular mass of 20.3 kDa. It migrates as an 21 kDa band in SDS-PAGE under reducing conditions as predicted.
Does Pin1 inhibition improve the efficacy of ralaniten compounds?
Leung JK et al. Pin1 inhibition improves the efficacy of ralaniten compounds that bind to the N-terminal domain of androgen receptor. Commun Biol 4:381 (2021).
How many amino acids are in human Pin1?
A DNA sequence encoding the mature form of human PIN1 (Q13526-1) (Met 1-Glu 163) was expressed, with a polyhistide tag at the N-terminus. The recombinant human PIN1 consisting of 179 amino acids and has a calculated molecular mass of 20.3 kDa.