What is alkaline picrate method?
Principle Picric acid in an alkaline medium reacts with creatinine to form a orange coloured complex with the alkaline picrate. Intensity of the colour formed is directly proportional to the amount of creatinine present in the sample.
What is Jaffe reaction for creatinine?
Creatinine in serum or urine is determined by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.
What interferes with the Jaffe reaction?
The Jaffe reaction is not specific for creatinine, the same reaction resulting in Jaffe-like chromogens show many interfering substances (ascorbic acid, bilirubin, proteins, ketones, cephalosporins and other drugs).
Is Jaffe an enzymatic reaction?
Background: The Jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. The Jaffe method is less expensive than the enzymatic method but is also more susceptible to interferences. Interferences can lead to misdiagnosis but interferences may vary by patient population.
What is Jaffe method?
The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine.
How do you make an alkaline picrate solution?
Alkaline picrate solution: Mix 5 volumes of saturated picric acid solution to one volume of NaOH. creatinine in 0.1 N HCl and diluted to 1 litre with acid. Working solutions : Dilute 3 ml of stock solution to 500 ml with water using 50 ml of 0.1 N HCl (0.006 mg/ml).
What is the Jaffe test?
How does the Jaffe method work?
Jaffe creatinine method is based on alkaline picrate. At an alkaline pH, creatinine in the sample reacts with picrate to form a creatinineâpicrate complex. The rate of increase in the absorbance at 500 nm because of the formation of this complex is directly proportional to the concentration of creatinine in the sample.
What substances will cause interference in the alkaline picrate method?
Slow-reacting substances such as protein, glucose and ascorbic acid can cause falsely high results by reducing alkaline picrate to picramate.
What is the difference between Jaffe and enzymatic creatinine?
Background. The Jaffe and enzymatic methods are the two most common methods for measuring serum creatinine. The Jaffe method is less expensive than the enzymatic method but is also more susceptible to interferences. Interferences can lead to misdiagnosis but interferences may vary by patient population.
What is the Jaffe method?
Who developed the alkaline picrate method for the determination of creatinine concentration?
Jaffe
Jaffe and the Jaffe principle In 1886, Jaffe observed that a red colour formed when creatinine reacted with picric acid in an alkaline medium [5] and observed the needle-formed crystals under the microscope.