What is the CB promoter?
The cytomegalovirus (CMV) early enhancer/promoter and the hybrid CMV enhancer/chicken β-actin (CBA) promoter are commonly used in gene transfer studies with both therapeutic and reporter genes. These promoters are typically used to provide robust, long-term expression in all cell types.
Does CMV promoter work in neurons?
Under a CMV promoter, gene expression is very poor • Under a Ubiquitin C promoter, gene expression is very high • CBh and synapsin promoters also work well in primary neurons. For neuroblastoma cell lines, such as Sh-SY5Y, SKNMC, SKNAS, KELLY, IMR32, LAN1, CMV promoter works well.
What is EFS promoter?
EF1alpha (or its short, intron-less form, EFS) is a promoter that has been recently used in many clinical trials. It is a cellular-derived enhancer/promoter with decreased cross-activation of nearby promoters, therefore hypothetically decreasing the risk of genotoxicity.
What is UBC promoter?
The promoter of the polyubiquitin C gene (UBC) contains putative heat shock elements (HSEs) which are thought to mediate UBC induction upon stress. However, the mapping and the functional characterization of the cis-acting determinants for its up-regulation have not yet been addressed.
How does promoter work?
Definition. A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated into a functional protein.
What activates CMV promoter?
Dimethylsulfoxide and 5-Aza-2′-deoxycytidine can activate the cytomegalovirus promoter in both cell types by overlapping mechanisms.
What binds to the CMV promoter?
The activation of the CMV promoter by Mekk1, on the other hand, is attributed to the proximal CRE binding site in the promoter. These findings may be of interest to investigators who use CMV (or other viral) promoter driven vectors for either comparative or quantitative gene expression, or effect on promoter activity.
What is a T7 promoter?
What Is the T7 Promoter Sequence? The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5′ – TAATACGACTCACTATAG – 3′) that is recognized by T7 RNA polymerase1 .
Is CMV a strong promoter?
While there is cell type to cell type variability for all the promoters, CMV promoter is the most variable, being very strong in some cell types (e.g., 293T and CMMT) and rather weak in others (e.g., MRC5 and MSC).
What is the weakest promoter?
UBC
It showed that UBC is consistently the weakest promoter in all the cell types while PGK is also consistently weak, though typically stronger than UBC. By contrast, EF1A and CAGG promoters are consistent strong in all the cell types, with EF1A being slightly stronger in some cell types and slightly weaker in others.