What is the sample volume if the blood is withdrawn from retro-orbital plexus in recovered rats?
The maximum amount of blood that may be withdrawn at one time from this location is 1% of the animal’s body weight (e.g., 0.2 ml from a 20 gm adult mouse and 2.0 ml’s from a 200 gm adult rat). Blood can only be collected once per week from one eye. Subsequent bleeds should use alternate eyes.
What is retro-orbital puncture?
Retro-Orbital Sinus Injection. • Is an injection into the ophthalmic venous. sinus (mice) or plexus (rats), or a retrobulbar.
How do you collect blood from mouse tail vein?
Tail vein sampling is suitable for a small volume of blood (less than 0.2 ml) by puncture (with a needle or lance) of the tail vein. It is suitable for all strains but is more difficult in black or pigmented mice. Multiple samples can be taken daily by alternating sides.
What topical anesthetic must be used when performing retro-orbital procedures?
Retro-orbital sampling in the rat must be conducted under general anesthesia. 2. Once anesthetized, apply a single drop of topical opthalmic anesthetic (Proparacaine Hydrochloride) prior to procedure. Allow a minimum of 30 seconds for the medication to take effect before collecting the blood sample.
How much blood can you take from a rat?
Single sample: Without fluid replacement, the maximum blood volume which can be safely removed for a one-time sample is 10% of the total blood volume or 5.5-7 ml/kg. With fluid replacement (LRS or sterile physiologic saline), up to 15% of the total blood volume or approximately 8.3-10.5 ml/kg can be removed.
What is the amount of blood collected at one time?
Blood sample may be collected over 24 hour at the volume of 0.1 to 0.2 ml/sample. After withdrawing the blood, the cannula is flushed with an anticoagulant and the withdrawn volume may be replaced (if required) with LRS and cannula should be closed tightly [Figure 5].
What is an eye orbit?
The orbit is the bony cavity in the skull that houses the globe of the eye (eyeball), the muscles that move the eye (the extraocular muscles), the lacrimal gland, and the blood vessels and nerves required to supply these structures.
How do you Cervically dislocate a mouse?
HCD was performed by placing a large hemostat behind the base of the anesthetized mouse’s skull and pulling back sharply on the tail. MCD was similar to HCD, except that thumb and forefingers were placed at the base of the skull instead of the hemostat.
Do mice bleed?
Under ideal conditions, a skilled person can obtain approximately 50-75% of a mouse’s total blood volume (3-4% of BW) by exsanguination. This amounts to 0.04-0.06 ml blood/gm BW, or 1.0-1.5 ml blood from a 25 gm mouse.
Which statement is true regarding anesthesia in mice?
Which statement is TRUE regarding anesthesia in mice? Gas anesthesia (using a vaporizer) is generally the best option for providing extended anesthesia.